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Professional brushcutters BC S. Professional brushcutters BC T. However, the level of production and the percentage of linoleic acid of the total fatty acids were very low in yeast.
In the same way as for yeast expression, several transformants were obtained. The fatty acid compositions of the A.
Therefore, glucose was used as a carbon source in the following experiments. Linoleic acid constituted As shown in Fig.
A single positive band 1. Genomic Southern blot analysis of Aspergillus transformants.
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The industrial strain M. In this strain, oleic acid can be successively desaturated to linoleic acid.
The evolutionary relationship of the fatty acid desaturases from M. Ltd, Tokyo, Japan is shown in Fig. Interestingly, the C. Phylogenetic tree for fatty acid desaturases.
Abbreviations are the same as in Fig. The accession numbers for the sequences arre: In general, it is difficult to express the genes of filamentous fungi in bacterial hosts such as E. Fungi, however, are suitable hosts for the expression of filamentous fungal genes [ 40 ]. This suggested that the phospholipids containing oleic acid a component of membrane bilayers in S.
As the amount of linoleic acid accumulated by the expression of the M. We used Aspergillus as the heterologous host for such expression, because this is one of the few filamentous fungi for which there is an effective host—vector system [ 40 ]. Our findings indicated that in future the genes encoding proteins involved in PUFA biosynthesis by M.
Furthermore, the oleaginous fungus, M. Continuous encouragement from Director Dr Chris R. Somerville Carnegie Institution of Washington, Stanford during the writing of this manuscript is gratefully acknowledged. Volume , Issue 3. If you do not receive an email within 10 minutes, your email address may not be registered, and you may need to create a new Wiley Online Library account. If the address matches an existing account you will receive an email with instructions to retrieve your username.
Open access. European Journal of Biochemistry Volume , Issue 3. Free Access. First published: Tools Request permission Export citation Add to favorites Track citation. Share Give access Share full text access. Share full text access.